Spore Preparation Protocol for Enrichment of Clostridia from Murine Intestine.
نویسندگان
چکیده
In recent years, many spore-forming commensal Clostridia found in the gut have been discovered to promote host physiology, immune development, and protection against infections. We provide a detailed protocol for rapid enrichment of spore-forming bacteria from murine intestine. Briefly, contents from the intestinal cecum are collected aerobically, diluted and finally treated with chloroform to enrich for Clostridia spores.
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ورودعنوان ژورنال:
- Bio-protocol
دوره 7 10 شماره
صفحات -
تاریخ انتشار 2017